IGEM:Peking/2007/Switch-Notebook/2007-8-15
From OpenWetWare
Mini-prep and double digestion of T1TE-pLX010, SS1-(GFP-pLX007)
T1TE-pLX010 ^^ with ScaI and XhoI
l to r: T1TE-pLX010^^1-22, 100bp ladder, lambda marker
Right 552bp/Wrong 512bp
Select for sequencing: 4, 8, 10, 11, 12, 14
SS1-(GFP-pLX007) ^^ with SalI and XhoI
l to r: SS1-(GFP-pLX007)^^1-5, 100bp ladder, lambda marker
Right 896bp/Wrong 756bp
All are wrong..
Colony PCR test of SulA, Rec, lac, SS1-3, SD1-3-(GFP-pLX007)
Fragment No. of colonies PCRed / selected ------------------------------------------------------------------------ SulA 24 / 4 lac 24 / 11 Rec 22 / 2 SS1 24 / 0 SS2 13 / 0 SS3 18 / 9 SD1 24 / 2 SD2 24 / 4 SD3 13 / 0