IGEM:Peking/2007/Switch-Notebook/2007-8-21: Difference between revisions
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==pcc009 digestion== | ==pcc009 digestion== | ||
XhoI and SalI | XhoI and SalI | ||
[[Image:PKU Switch 07-8-21 pcc009 digestion.jpg]] | [[Image:PKU Switch 07-8-21 pcc009 digestion.jpg]] | ||
the left one is pcc009-1, 0.25uL enzyme/20uL | |||
the right one is pcc009-3, 2uL enzyme/100uL | |||
Latest revision as of 20:50, 26 August 2007
precipitation of plx012
colony PCR to test lacI-pcc009(old)
no one seems to be right!
re-ligation of pcc009 and lacIq
transformation tonight
re-PCR lacIq
re-PCR ss1, rs1, rs2, rd2, rd3, cd1
from left to right is CD1, SS1, RD2. RD3, RD3, RS1, RS2
lacI double digestion
lacI 10uL buffer H 10uL XhoI 2uL SalI 2uL ddH2O 76uL total 100uL
pcc009 digestion
XhoI and SalI
the left one is pcc009-1, 0.25uL enzyme/20uL the right one is pcc009-3, 2uL enzyme/100uL
