IGEM:Peking/2007/Switch-Notebook/2007-8-22: Difference between revisions
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Chen Chongyi (talk | contribs) (New page: ==organize double promoter== we have already got 9 promoters with prove of sequencing: 2 Rec, 1 lac, 3 SS1, 2 SS2, 2 SS3, 4 SD2, 1 SD3, 2 RD1 and 1 RS3 we still need 8 kinds of promoters: ...) |
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==colony PCR to test lacI-pcc009== | ==colony PCR to test lacI-pcc009== | ||
primers are pkan-lam-F and pcc-test-R | primers are pkan-lam-F and pcc-test-R | ||
[[Image:PKU Switch 07-8- | |||
[[Image:PKU Switch 07-8- | [[Image:PKU Switch 07-8-22 lacI-pcc009 1.jpg]] | ||
[[Image:PKU Switch 07-8- | |||
[[Image:PKU Switch 07-8-22 lacI-pcc009 2.jpg]] | |||
[[Image:PKU Switch 07-8-22 lacI-pcc009 3.jpg]] | |||
Latest revision as of 19:52, 26 August 2007
organize double promoter
we have already got 9 promoters with prove of sequencing: 2 Rec, 1 lac, 3 SS1, 2 SS2, 2 SS3, 4 SD2, 1 SD3, 2 RD1 and 1 RS3 we still need 8 kinds of promoters: sulA, SD1, RD2, RD3, RS1, RS2, CD1 and CD2
re-PCR CD1, CD2 and SulA
plasmid mini-prep of lacI-pcc009
re-ligation of lacI-PMD18-T and transformation
ligation of SS1, RS1, RS2, RD2, lacI with pcc009
colony PCR to test lacI-pcc009
primers are pkan-lam-F and pcc-test-R
