IGEM:Peking/2007/Switch-Notebook/2007-8-26: Difference between revisions
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==colony PCR to check any successful ligation== | ==colony PCR to check any successful ligation== | ||
==ligation of plx012 with CD2, sulA, RS2 and RD3== | ==ligation of plx012 with CD2, sulA, RS2 and RD3== | ||
fragment 4uL, ligation kit 5uL, plasmid 1uL | |||
overnight under 4 degree | |||
Revision as of 08:26, 26 August 2007
colony PCR to check any successful ligation
ligation of plx012 with CD2, sulA, RS2 and RD3
fragment 4uL, ligation kit 5uL, plasmid 1uL overnight under 4 degree
