IGEM:Peking/2007/Switch-Notebook/2007-8-26: Difference between revisions
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==colony PCR to check any successful ligation between lacIq and pcc009== | ==colony PCR to check any successful ligation between lacIq and pcc009== | ||
[[Image:PKU Switch 07-8-26 lacI-pcc009_1.gif]] | |||
==ligation of plx012 with CD2, sulA, RS2 and RD3== | ==ligation of plx012 with CD2, sulA, RS2 and RD3== | ||
fragment 4uL, ligation kit 5uL, plasmid 1uL | fragment 4uL, ligation kit 5uL, plasmid 1uL | ||
overnight under 4 degree | overnight under 4 degree | ||
Revision as of 19:13, 26 August 2007
colony PCR to check any successful ligation between lacIq and pcc009
File:PKU Switch 07-8-26 lacI-pcc009 1.gif
ligation of plx012 with CD2, sulA, RS2 and RD3
fragment 4uL, ligation kit 5uL, plasmid 1uL overnight under 4 degree
