IGEM:Peking/2007/Switch-Notebook/2007-8-26: Difference between revisions
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==colony PCR to check any successful ligation between lacIq and pcc009== | ==colony PCR to check any successful ligation between lacIq and pcc009== | ||
[[Image:PKU Switch 07-8-26 lacI-pcc009_1. | [[Image:PKU Switch 07-8-26 lacI-pcc009_1.jpg]] | ||
==ligation of plx012 with CD2, sulA, RS2 and RD3== | ==ligation of plx012 with CD2, sulA, RS2 and RD3== | ||
fragment 4uL, ligation kit 5uL, plasmid 1uL | fragment 4uL, ligation kit 5uL, plasmid 1uL | ||
overnight under 4 degree | overnight under 4 degree | ||
Revision as of 19:26, 26 August 2007
colony PCR to check any successful ligation between lacIq and pcc009
ligation of plx012 with CD2, sulA, RS2 and RD3
fragment 4uL, ligation kit 5uL, plasmid 1uL overnight under 4 degree
