IGEM:Peking/2007/Switch-Notebook/2007-8-26: Difference between revisions
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experimenter:JLL, LC | |||
==colony PCR to check any successful ligation between lacIq and pcc009== | ==colony PCR to check any successful ligation between lacIq and pcc009== | ||
[[Image:PKU Switch 07-8-26 lacI-pcc009_1.jpg]] | [[Image:PKU Switch 07-8-26 lacI-pcc009_1.jpg]] | ||
one lane seems to be right, be sequenced tomorrow | one lane seems to be right, be sequenced tomorrow | ||
==double promoters colony PCR== | |||
[[Image:PKU Switch 07-8-26 double promoters.jpg]] | |||
there are some loading problems after 10th lane | |||
==colony PCR of lacI-pcc009== | |||
easy taq 0.1uL | |||
pkan-F 0.5uL | |||
pcc-test-R 0.5uL | |||
buffer 2uL | |||
dNTP 2uL | |||
ddH2O 15uL | |||
[[Image:PKU Switch 07-8-26 lacI-pcc009.jpg]] | |||
1-9: lacI-pcc009, all wrong | |||
10: self-ligation control | |||
11-13: positive transformation lacI-pcc009 | |||
==ligation of plx012 with CD2, sulA, RS2 and RD3== | ==ligation of plx012 with CD2, sulA, RS2 and RD3== | ||
fragment 4uL, ligation kit 5uL, plasmid 1uL | fragment 4uL, ligation kit 5uL, plasmid 1uL | ||
overnight under 4 degree | overnight under 4 degree | ||
Latest revision as of 00:45, 27 August 2007
experimenter:JLL, LC
colony PCR to check any successful ligation between lacIq and pcc009
one lane seems to be right, be sequenced tomorrow
double promoters colony PCR
there are some loading problems after 10th lane
colony PCR of lacI-pcc009
easy taq 0.1uL pkan-F 0.5uL pcc-test-R 0.5uL buffer 2uL dNTP 2uL ddH2O 15uL
1-9: lacI-pcc009, all wrong 10: self-ligation control 11-13: positive transformation lacI-pcc009
ligation of plx012 with CD2, sulA, RS2 and RD3
fragment 4uL, ligation kit 5uL, plasmid 1uL overnight under 4 degree
