IGEM:Peking/2007/Switch-Notebook/2007-8-26
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colony PCR to check any successful ligation between lacIq and pcc009
one lane seems to be right, be sequenced tomorrow
double promoters colony PCR
there are some loading problems after 10th lane
colony PCR of lacI-pcc009
easy taq 0.1uL pkan-F 0.5uL pcc-test-R 0.5uL buffer 2uL dNTP 2uL ddH2O 15uL
1-9: lacI-pcc009, all wrong 10: self-ligation control 11-13: positive transformation lacI-pcc009
ligation of plx012 with CD2, sulA, RS2 and RD3
fragment 4uL, ligation kit 5uL, plasmid 1uL overnight under 4 degree