IGEM:Peking/2007/Switch-Notebook/2007-8-26

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experimenter:JLL, LC

colony PCR to check any successful ligation between lacIq and pcc009

one lane seems to be right, be sequenced tomorrow

double promoters colony PCR

there are some loading problems after 10th lane

colony PCR of lacI-pcc009

 easy taq 0.1uL
 pkan-F 0.5uL
 pcc-test-R 0.5uL
 buffer 2uL
 dNTP 2uL
 ddH2O 15uL

1-9: lacI-pcc009, all wrong 10: self-ligation control 11-13: positive transformation lacI-pcc009

ligation of plx012 with CD2, sulA, RS2 and RD3

  fragment 4uL, ligation kit 5uL, plasmid 1uL
  overnight under 4 degree
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