IGEM:Peking/2007/Switch-Notebook/2007-8-28: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Chen Chongyi (talk | contribs) (New page: ==Colony PCR of CD2, SulA, RS2, RD3 ligated with plx012== buffer 1.5uL dNTP 1.5uL dH2O 11uL test-F/GFP-test-R 0.5uL each easy taq 0.1uL elongation time span of 1 minute ==T1T2-pcc01...) |
Chen Chongyi (talk | contribs) |
||
| Line 18: | Line 18: | ||
==transformation== | ==transformation== | ||
CD2, sulA, RS2, RD3-plx012 | CD2, sulA, RS2, RD3-plx012 | ||
positive transformation: CD1-plx012 and plx012 itself | positive transformation: CD1-plx012 and plx012 itself | ||
Latest revision as of 09:43, 26 October 2007
Colony PCR of CD2, SulA, RS2, RD3 ligated with plx012
buffer 1.5uL
dNTP 1.5uL
dH2O 11uL
test-F/GFP-test-R 0.5uL each
easy taq 0.1uL
elongation time span of 1 minute
T1T2-pcc010 rescue by precipitation
T1TE-pMD18T rescue by gel eletrophoresis
transformation
CD2, sulA, RS2, RD3-plx012
positive transformation: CD1-plx012 and plx012 itself
plx012 and CD1-plx012 culivated in advance
miniprep of plx012 and CD1-plx012
double digestion test to prove its accuracy.
