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Colony PCR of T1TE-T1T2-pcc010

primer: pcctestR and pkan-Kan-F

some colonies may be right!

Colony PCR of 434OR/21/321

primer: MBF and MBR

some colonies may be right, send to sequence.

Colony PCR of CD-plx012

some colonies may be right!

rescue by gel of I13521

I7100 miniprep after picking some colonies

digestion test afterwards using EcoRI/PstI in H buffer, overnight

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