IGEM:Peking/2007/Switch-Notebook/2007-8-7: Difference between revisions
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Chen Daizhuo (talk | contribs) (New page: ==Ligation and transformation== <pre> T1TE^^(A/X) lacZa-pLX003^^ lacZ-pLX003^^(A/X) (self-ligation-control) </pre> ==Digestion of plasmids== <pre> 2uL T1T2-pCC002 2uL C...) |
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Latest revision as of 19:34, 18 August 2007
Ligation and transformation
T1TE^^(A/X) lacZa-pLX003^^ lacZ-pLX003^^(A/X) (self-ligation-control)
Digestion of plasmids
2uL T1T2-pCC002 2uL ClaI 2uL SalI 10uL 10*H buffer 84uL H2O ---------------------- 100uL total
2uL SDY-EGFP=pLX007 2uL AatII 2uL XhoI 10uL T 10uL BSA 74uL H2O ---------------------- 100uL total
