Precipitation of EGFP, 434OR21/321
rescue them afterwards and run a gel to check
PCR double repressors
This is a second time, just in case we used up all the products last time
Miniprep of T1TE-T1T2-pcc010
digestion test using ClaI and ScaI in H buffer, but failed
I am sure the plasmid is right, next I will change some other endonucleae to see!
PCR of parts for switch the third time
This time we have rescued most of the parts we need in the future!
Together with the first 2 times, we got every parts for at least once.
ligation of I7100 with SDY-EGFP, 434OR321/21
Transformation afterwards and overnight culivation