From OpenWetWare

< IGEM:Peking | 2007
Revision as of 20:45, 26 October 2007 by Chen Chongyi (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search


Check our plate

By smearing competent cells directly onto plates without any transformation procedure, we can check whether the plates with Kanamycin is right.

Result: The plates have some thing wrong and we discarded them!

Colony PCR of I7100 ligation product


I7100 digestion test

using H buffer, PstI/EcoRI


Digestion of cIind-

digestion another time of vector and fragments

using still PstI/EcoRI and H buffer

Miniprep of I7100 for the second time

Personal tools