IGEM:Peking/2007/Switch: DNA ligation: Difference between revisions
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Chen Chongyi (talk | contribs) (New page: ==连接== 1.普通连接的体系为10uL,其中片断+载体5uL,Ligation Kit 5uL。 2.Ligation Kit需在冰盒中溶化。分装至1.5mL离心管中使用。 3.先加片断...) |
Chen Chongyi (talk | contribs) (→连接) |
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== | ==DNA ligation system== | ||
1.10uL, fragment+ vector 5uL, Ligation Kit 5uL. | |||
2.Ligation | 2.Ligation Kit should be separately loaded into 1.5mL EP tubes for any usage. | ||
3.First add the fragment, then add the vector. | |||
4.cover the tube cap using parafilm. | |||
5.16℃ reaction more than 1 hour. | |||
Latest revision as of 05:17, 20 October 2007
DNA ligation system
1.10uL, fragment+ vector 5uL, Ligation Kit 5uL.
2.Ligation Kit should be separately loaded into 1.5mL EP tubes for any usage.
3.First add the fragment, then add the vector.
4.cover the tube cap using parafilm.
5.16℃ reaction more than 1 hour.