IGEM:Peking/2007/Switch: DNA rescue by precipitation

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DNA沉淀回收

1. 向体系中加入2倍体积的100%乙醇,1/10体积的3M NaAC,放置适当时间可使DNA沉淀。对于载体,一般不短于30min。片断越短需要沉淀时间越长。<100bp的片断则应用等体积的异丙醇+1/10体积的3M NaAC沉淀过夜。

2. 12,000rpm离心15min。

3. 弃去上清。

4. 加入200uL 70%乙醇清洗管壁。

5. 12,000rpm离心10min。

6. 弃上清。

7. 在37℃烘干。

8. 溶于适当体积(20uL)的ddH2O中。

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