IGEM:Peking University/2008/Experiment/PKU 0603 G2 1: Difference between revisions
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==PCR== | ==PCR== | ||
{|border="1" cellspacing="0" | *{|border="1" cellspacing="0" | ||
|+ '''TITLE: PCR-Budding Yeast Genome-Gal4-Easytaq 20ul*6''' | |+ '''TITLE: PCR-Budding Yeast Genome-Gal4-Easytaq 20ul*6''' | ||
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| Line 45: | Line 45: | ||
|} | |} | ||
{|border="1" cellspacing="0" | *{|border="1" cellspacing="0" | ||
|+ '''TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*3''' | |+ '''TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*3''' | ||
|- | |- | ||
Revision as of 09:36, 3 July 2008
PCR
- {|border="1" cellspacing="0"
|+ TITLE: PCR-Budding Yeast Genome-Gal4-Easytaq 20ul*6 |- | ||Volume (μL) |- |Template （Budding Yeast Genome）||1 |- |dNTP||2 |- |Enzyme||0.5 |- |Buffer||2 |- |F-Gal4-HindIII-ApaI ||0.5 |- |R-Gal4-EcoRI-Xhol||0.5 |- |ddH2O||13.5 |}
- conditions
| Temperature(&&&) | Time Length(0:00:00) | |
| 94°C | 02:00 | |
| 94°C | 01:00 | 4 cycles |
| 50°C | 02:00 | 4 cycles |
| 72°C | 00:30 | 4 cycles |
| 94°C | 01:00 | 30 cycles |
| 52.0,53.6,56.1,58.8,60.1,62.0°C | 02:00 | 30 cycles |
| 72°C | 04:00 | 30 cycles |
| 72°C | 05:00 | |
| 4°C | Hold |
- {|border="1" cellspacing="0"
|+ TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*3 |- | ||Volume (μL) |- |Template （Budding Yeast Genome）||1 |- |dNTP||5 |- |Enzyme||1 |- |Buffer||5 |- |F-Gal4-HindIII-ApaI ||1 |- |R-Gal4-EcoRI-Xhol||1 |- |ddH2O||36 |}
- conditions
| Temperature | Time Length(0:00:00) | |
| 94°C | 02:00 | |
| 94°C | 01:00 | 4 cycles |
| 50°C | 02:00 | 4 cycles |
| 72°C | 00:30 | 4 cycles |
| 94°C | 01:00 | 30 cycles |
| 54.7,57.4,60.1°C | 02:00 | 30 cycles |
| 72°C | 04:00 | 30 cycles |
| 72°C | 05:00 | |
| 4°C | Hold |
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