IGEM:Peking University/2008/Experiment/PKU 0603 G2 1: Difference between revisions
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==PCR== | ==PCR== | ||
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|+ '''TITLE: PCR-Budding Yeast Genome-Gal4-Easytaq 20ul*6''' | |+ '''TITLE: PCR-Budding Yeast Genome-Gal4-Easytaq 20ul*6''' | ||
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|+ '''TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*3''' | |+ '''TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*3''' | ||
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Revision as of 09:36, 3 July 2008
PCR
| Volume (μL) | |
| Template （Budding Yeast Genome） | 1 |
| dNTP | 2 |
| Enzyme | 0.5 |
| Buffer | 2 |
| F-Gal4-HindIII-ApaI | 0.5 |
| R-Gal4-EcoRI-Xhol | 0.5 |
| ddH2O | 13.5 |
- conditions
| Temperature(&&&) | Time Length(0:00:00) | |
| 94°C | 02:00 | |
| 94°C | 01:00 | 4 cycles |
| 50°C | 02:00 | 4 cycles |
| 72°C | 00:30 | 4 cycles |
| 94°C | 01:00 | 30 cycles |
| 52.0,53.6,56.1,58.8,60.1,62.0°C | 02:00 | 30 cycles |
| 72°C | 04:00 | 30 cycles |
| 72°C | 05:00 | |
| 4°C | Hold |
| Volume (μL) | |
| Template （Budding Yeast Genome） | 1 |
| dNTP | 5 |
| Enzyme | 1 |
| Buffer | 5 |
| F-Gal4-HindIII-ApaI | 1 |
| R-Gal4-EcoRI-Xhol | 1 |
| ddH2O | 36 |
- conditions
| Temperature | Time Length(0:00:00) | |
| 94°C | 02:00 | |
| 94°C | 01:00 | 4 cycles |
| 50°C | 02:00 | 4 cycles |
| 72°C | 00:30 | 4 cycles |
| 94°C | 01:00 | 30 cycles |
| 54.7,57.4,60.1°C | 02:00 | 30 cycles |
| 72°C | 04:00 | 30 cycles |
| 72°C | 05:00 | |
| 4°C | Hold |
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