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PCR
TITLE: PCR-Budding Yeast Genome-Gal4-Easytaq 20ul*6
|
Volume (μL)
|
| Template （Budding Yeast Genome） |
1
|
| dNTP |
2
|
| Enzyme |
0.5
|
| Buffer |
2
|
| F-Gal4-HindIII-ApaI |
0.5
|
| R-Gal4-EcoRI-Xhol |
0.5
|
| ddH2O |
13.5
|
- conditions
| Temperature(&&&) |
Time Length(0:00:00)
|
| 94°C |
02:00
|
| 94°C |
01:00 |
4 cycles
|
| 50°C |
02:00 |
4 cycles
|
| 72°C |
00:30 |
4 cycles
|
| 94°C |
01:00 |
30 cycles
|
| 52.0,53.6,56.1,58.8,60.1,62.0°C |
02:00 |
30 cycles
|
| 72°C |
04:00 |
30 cycles
|
| 72°C |
05:00
|
| 4°C |
Hold
|
TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*3
|
Volume (μL)
|
| Template （Budding Yeast Genome） |
1
|
| dNTP |
5
|
| Enzyme |
1
|
| Buffer |
5
|
| F-Gal4-HindIII-ApaI |
1
|
| R-Gal4-EcoRI-Xhol |
1
|
| ddH2O |
36
|
- conditions
| Temperature |
Time Length(0:00:00)
|
| 94°C |
02:00
|
| 94°C |
01:00 |
4 cycles
|
| 50°C |
02:00 |
4 cycles
|
| 72°C |
00:30 |
4 cycles
|
| 94°C |
01:00 |
30 cycles
|
| 54.7,57.4,60.1°C |
02:00 |
30 cycles
|
| 72°C |
04:00 |
30 cycles
|
| 72°C |
05:00
|
| 4°C |
Hold
|
