IGEM:Peking University/2008/Experiment/PKU 0605 G2 1: Difference between revisions
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(New page: ::'''Enzyme cutting-pUC18-HindIII-EcoRI-20 μL''' {|align="right" 400px |} {|border="1" cellspacing="0" |+ |- | ||Volumn(μL) |- | pUC18 ||5 |- |...) |
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::'''Enzyme cutting-pUC18-HindIII-EcoRI-20 μL''' | ::'''Enzyme cutting-pUC18-HindIII-EcoRI-20 μL''' | ||
{|border="1" cellspacing="0" | {|border="1" cellspacing="0" | ||
|+ | |+ | ||
| Line 12: | Line 9: | ||
| HindIII||0.2 | | HindIII||0.2 | ||
|- | |- | ||
| EcoRI||0.2 | | EcoRI||0.2 | ||
|- | |- | ||
| M Buffer||2 | | M Buffer||2 | ||
| Line 18: | Line 15: | ||
|ddH<sub>2</sub>O||12.6 | |ddH<sub>2</sub>O||12.6 | ||
|} | |} | ||
<hr class=divider> | |||
::'''Enzyme cutting-Gal4-HindIII-EcoRI-50 μL''' | |||
{|border="1" cellspacing="0" | |||
|+ | |||
|- | |||
| ||Volumn(μL) | |||
|- | |||
| Gal4||15 | |||
|- | |||
| HindIII||0.2 | |||
|- | |||
| EcoRI||0.2 | |||
|- | |||
| M Buffer||5 | |||
|- | |||
|ddH<sub>2</sub>O||29 | |||
|} | |||
<hr class=divider> | |||
::''' Ligation-pUC18/Gal4''' | |||
{|align="right" | {|align="right" | ||
|back to [[PKU_Experiment|Previous Page]] | |back to [[PKU_Experiment|Previous Page]] | ||
|} | |} | ||
Latest revision as of 21:49, 11 July 2008
- Enzyme cutting-pUC18-HindIII-EcoRI-20 μL
| Volumn(μL) | |
| pUC18 | 5 |
| HindIII | 0.2 |
| EcoRI | 0.2 |
| M Buffer | 2 |
| ddH2O | 12.6 |
- Enzyme cutting-Gal4-HindIII-EcoRI-50 μL
| Volumn(μL) | |
| Gal4 | 15 |
| HindIII | 0.2 |
| EcoRI | 0.2 |
| M Buffer | 5 |
| ddH2O | 29 |
- Ligation-pUC18/Gal4
| back to Previous Page |
