IGEM:Peking University/2008/Experiment/PKU 0605 G2 1: Difference between revisions
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::'''Enzyme cutting-Gal4-HindIII-EcoRI- | ::'''Enzyme cutting-Gal4-HindIII-EcoRI-50 μL''' | ||
{|border="1" cellspacing="0" | {|border="1" cellspacing="0" | ||
|+ | |+ | ||
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| ||Volumn(μL) | | ||Volumn(μL) | ||
|- | |- | ||
| | | Gal4||15 | ||
|- | |- | ||
| | | HindIII||0.2 | ||
|- | |- | ||
| | | EcoRI||0.2 | ||
|- | |- | ||
| M Buffer|| | | M Buffer||5 | ||
|- | |- | ||
|ddH<sub>2</sub>O|| | |ddH<sub>2</sub>O||29 | ||
|} | |} | ||
<hr class=divider> | |||
::''' Ligation-pUC18/Gal4''' | |||
{|align="right" | {|align="right" | ||
|back to [[PKU_Experiment|Previous Page]] | |back to [[PKU_Experiment|Previous Page]] | ||
|} | |} | ||
Latest revision as of 21:49, 11 July 2008
- Enzyme cutting-pUC18-HindIII-EcoRI-20 μL
| Volumn(μL) | |
| pUC18 | 5 |
| HindIII | 0.2 |
| EcoRI | 0.2 |
| M Buffer | 2 |
| ddH2O | 12.6 |
- Enzyme cutting-Gal4-HindIII-EcoRI-50 μL
| Volumn(μL) | |
| Gal4 | 15 |
| HindIII | 0.2 |
| EcoRI | 0.2 |
| M Buffer | 5 |
| ddH2O | 29 |
- Ligation-pUC18/Gal4
| back to Previous Page |
