IGEM:Peking University/2008/Experiment/PKU 0607 G2 1: Difference between revisions
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Latest revision as of 21:49, 11 July 2008
Volume (μL) | |
Template (Budding Yeast Genome) | 1 |
dNTP | 5 |
Enzyme | 1 |
Buffer | 5 |
F-Gal4-HindIII-ApaI | 1 |
R-Gal4-EcoRI-Xhol | 1 |
ddH2O | 36 |
- conditions
Temperature | Time Length(0:00:00) | |
94°C | 02:00 | |
94°C | 01:00 | 4 cycles |
50°C | 02:00 | 4 cycles |
72°C | 00:30 | 4 cycles |
94°C | 01:00 | 30 cycles |
49.2,52.6,54.6,56.5,58.2,59.8°C | 02:00 | 30 cycles |
72°C | 04:00 | 30 cycles |
72°C | 05:00 | |
4°C | Hold |
- Enzyme cutting-pUC18&Gal4-HindIII-EcoRI-20 μL
Volumn(μL) | |
pUC18 | 5 |
HindIII | 0.2 |
EcoRI | 0.2 |
M Buffer | 2 |
ddH2O | 12.6 |
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