IGEM:Peking University/2008/Experiment/PKU 0623 G2 1: Difference between revisions
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(New page: {|border="1" cellspacing="0" |+ '''TITLE: PCR-pPT-Gal4-Transtart 50ul*2''' |- | ||Volume (μL) |- |Template||1 |- |dNTP||5 |- |Enzyme||0.5 |- |Buffer||5 |- |F-Primer||0.5 |- |R-Prime...) |
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(No difference)
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Latest revision as of 21:50, 11 July 2008
| Volume (μL) | |
| Template | 1 |
| dNTP | 5 |
| Enzyme | 0.5 |
| Buffer | 5 |
| F-Primer | 0.5 |
| R-Primer | 0.5 |
| ddH2O | 37.5 |
- conditions
| Temperature | Time Length(0:00:00) | |
| 94°C | 02:00 | |
| 94°C | 01:00 | 4 cycles |
| 50°C | 02:00 | 4 cycles |
| 72°C | 05:00 | 4 cycles |
| 94°C | 01:00 | 30 cycles |
| 57.4,58.8°C | 01:00 | 30 cycles |
| 72°C | 03:00 | 30 cycles |
| 72°C | 10:00 | |
| 4°C | Hold |
| back to Previous Page |
