IGEM:Peking University/2008/Experiment/PKU 0623 G2 1

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TITLE: PCR-pPT-Gal4-Transtart 50ul*2
Volume (μL)
Template1
dNTP5
Enzyme0.5
Buffer5
F-Primer0.5
R-Primer0.5
ddH2O37.5
conditions
TemperatureTime Length(0:00:00)
94°C02:00
94°C01:004 cycles
50°C02:004 cycles
72°C05:004 cycles
94°C01:0030 cycles
57.4,58.8°C01:0030 cycles
72°C03:0030 cycles
72°C10:00
4°CHold
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