IGEM:Peking University/2008/Experiment/PKU 0701 G2 1: Difference between revisions

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{|border="1" cellspacing="0"
|+ '''TITLE: PCR-pPT-Gal4-Transtart  50ul*6'''
|-
| ||Volume (μL)
|-
|Template||1
|-
|dNTP||5
|-
|Enzyme||0.5
|-
|Buffer||5
|-
|F-Primer||0.5
|-
|R-Primer||0.5
|-
|ddH<sub>2O</sub>||37.5
|}


::'''conditions'''
{|border="1" cellspacing="0"
|+
|-
|Temperature||Time Length(0:00:00)
|-
| 94°C||02:00
|-
| 94°C||01:00||4 cycles
|-
| 50°C||02:00||4 cycles
|-
| 72°C||05:00||4 cycles
|-
| 94°C||01:00||30 cycles
|-
| 55.2,55.6,57.6,59.5,61.3,63.9°C||01:00||30 cycles
|-
| 72°C||03:00||30 cycles
|-
| 72°C||10:00
|-
| 4°C||Hold
|}
<hr class=divider>
{|align="right"
|back to [[PKU_Experiment|Previous Page]]
|}

Revision as of 06:37, 4 July 2008

TITLE: PCR-pPT-Gal4-Transtart 50ul*6
Volume (μL)
Template 1
dNTP 5
Enzyme 0.5
Buffer 5
F-Primer 0.5
R-Primer 0.5
ddH2O 37.5
conditions
Temperature Time Length(0:00:00)
94°C 02:00
94°C 01:00 4 cycles
50°C 02:00 4 cycles
72°C 05:00 4 cycles
94°C 01:00 30 cycles
55.2,55.6,57.6,59.5,61.3,63.9°C 01:00 30 cycles
72°C 03:00 30 cycles
72°C 10:00
4°C Hold


back to Previous Page
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