IGEM:Peking University/2008/Experiment/PKU 0701 G2 1: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 44: | Line 44: | ||
|} | |} | ||
<hr class=divider> | <hr class=divider> | ||
::'''Enzyme cutting-T-Gal4-EcoRI-HindIII-20*4 μL''' | |||
{|border="1" cellspacing="0" | |||
|+ | |||
|- | |||
| ||Volumn(μL) | |||
|- | |||
| DNA||5 | |||
|- | |||
| EcoRI||0.2 | |||
|- | |||
| HindIII||0.2 | |||
|- | |||
| M Buffer||2 | |||
|- | |||
|ddH<sub>2</sub>O||12.6 | |||
|} | |||
<hr class=divider> | |||
::''' Ligation-pUC18/Gal4''' | |||
{|align="right" | {|align="right" | ||
|back to [[PKU_Experiment|Previous Page]] | |back to [[PKU_Experiment|Previous Page]] | ||
|} | |} | ||
Revision as of 06:39, 4 July 2008
| Volume (μL) | |
| Template | 1 |
| dNTP | 5 |
| Enzyme | 0.5 |
| Buffer | 5 |
| F-Primer | 0.5 |
| R-Primer | 0.5 |
| ddH2O | 37.5 |
- conditions
| Temperature | Time Length(0:00:00) | |
| 94°C | 02:00 | |
| 94°C | 01:00 | 4 cycles |
| 50°C | 02:00 | 4 cycles |
| 72°C | 05:00 | 4 cycles |
| 94°C | 01:00 | 30 cycles |
| 55.2,55.6,57.6,59.5,61.3,63.9°C | 01:00 | 30 cycles |
| 72°C | 03:00 | 30 cycles |
| 72°C | 10:00 | |
| 4°C | Hold |
- Enzyme cutting-T-Gal4-EcoRI-HindIII-20*4 μL
| Volumn(μL) | |
| DNA | 5 |
| EcoRI | 0.2 |
| HindIII | 0.2 |
| M Buffer | 2 |
| ddH2O | 12.6 |
- Ligation-pUC18/Gal4
| back to Previous Page |
