IGEM:Peking University/2008/Experiment/PKU 0701 G2 1

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TITLE: PCR-pPT-Gal4-Transtart 50ul*6
Volume (μL)
Template1
dNTP5
Enzyme0.5
Buffer5
F-Primer0.5
R-Primer0.5
ddH2O37.5
conditions
TemperatureTime Length(0:00:00)
94°C02:00
94°C01:004 cycles
50°C02:004 cycles
72°C05:004 cycles
94°C01:0030 cycles
55.2,55.6,57.6,59.5,61.3,63.9°C01:0030 cycles
72°C03:0030 cycles
72°C10:00
4°CHold

Enzyme cutting-T-Gal4-EcoRI-HindIII-20*4 μL
Volumn(μL)
DNA5
EcoRI0.2
HindIII0.2
M Buffer2
ddH2O12.6

Ligation-pUC18/Gal4
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