IGEM:Peking University/2008/Experiment/PKU 0716 G3 1: Difference between revisions
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[[Image:PKU-20080716-Clony PCR-AH109+pGREG503-lacI.tif|center| | [[Image:PKU-20080716-Clony PCR-AH109+pGREG503-lacI.tif|center|400px]] | ||
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Latest revision as of 08:31, 17 July 2008
PKU_080716_G3_1
- Colony PCR-AH109+pGREG503-LacI
- PCR system 15μL
Volume (μL) | |
buffer | 5 |
Yeast | a few |
dNTP | 1.5 |
F-Primer | 0.3 |
R-Primer | 0.3 |
ddH2O | 11.4 |
- conditions
Temperature(°C) | Time Length(0:00:00) | |
98 | 05:00 | |
94 | 00:30 | 35 cycles |
60 | 00:30 | 35 cycles |
72 | 02:30 | 35 cycles |
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