IGEM:Peking University/2008/Experiment/PKU 080512 G1 1: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Jiqian Xing (talk | contribs) |
Jiqian Xing (talk | contribs) |
||
Line 72: | Line 72: | ||
|- | |- | ||
| Hold||4°C | | Hold||4°C | ||
|} | |||
{|align="right" | |||
|back to [[PKU_Experiment|Previous Page]] | |||
|} | |} |
Revision as of 09:42, 3 July 2008
PKU_080512_G1_1
- Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
Volume (μL) | |
DNA | 5 |
ClaI | 0.2 |
BamH1 | 0.2 |
KBuffer | 2 |
ddH2O | 12.6 |
- PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
Volume (μL) | |
pGREG505/(TEF-lacI-adhlacS-GFP) | 5 |
dNTP | 5 |
KOD Plus | 1 |
KOD Plus Buffer | 5 |
MgSO4 | 2 |
F-Primer | 1 |
R-Primer | 1 |
ddH2O | 34 |
- conditions
Temperature(°C) | Time Length(0:00:00) | |
94 | 05:00 | |
94 | 00:30 | 30 cycles |
55 | 00:30 | 30 cycles |
72 | 01:30 | 30 cycles |
72 | 07:00 | |
Alarm | 5 times | |
Hold | 4°C |
back to Previous Page |