IGEM:Peking University/2008/Experiment/PKU 080512 G1 1

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(PKU_080512_G1_1)
(PKU_080512_G1_1)
Line 72: Line 72:
|-
|-
| Hold||4°C
| Hold||4°C
 +
|}
 +
 +
{|align="right"
 +
|back to [[PKU_Experiment|Previous Page]]
|}
|}

Revision as of 12:42, 3 July 2008

PKU_080512_G1_1


Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
200px
Volume (μL)
DNA5
ClaI0.2
BamH10.2
KBuffer2
ddH2O 12.6



PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
200px
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP)5
dNTP5
KOD Plus1
KOD Plus Buffer5
MgSO42
F-Primer1
R-Primer1
ddH2O34


conditions
Temperature(°C)Time Length(0:00:00)
9405:00
9400:3030 cycles
5500:3030 cycles
7201:3030 cycles
7207:00
Alarm5 times
Hold4°C
back to Previous Page
Personal tools