IGEM:Peking University/2008/Experiment/PKU 080512 G1 1

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(New page: ==PKU_080512_G1_1== ::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA''' {|align="right" 200px |} {|border="1" cellspacing="0" |+ |- | ||Volume (μL) ...)
Current revision (00:46, 12 July 2008) (view source)
 
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==PKU_080512_G1_1==
==PKU_080512_G1_1==
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<hr class=divider>
::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA'''
::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA'''
{|align="right"
{|align="right"
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[[PKU_photo1.jpg|200px]]
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[[Image:PKU_080512_ADH-plasmid-check(95min).tif|400px]]
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::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
{|align="right"
{|align="right"
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[[PKU_20080512_adhpcrcheck.tif|200px]]
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[[Image:PKU_080512_ADH-PCR-check.tif|200px]]
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|Temperature(&&&)||Time Length(0:00:00)
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|Temperature(°C)||Time Length(0:00:00)
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| 94||05:00
| 94||05:00
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| Alarm||5 times
| Alarm||5 times
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| Hold||4&&&
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| Hold||4°C
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|back to [[PKU_Experiment|Previous Page]]
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Current revision

PKU_080512_G1_1


Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
Volume (μL)
DNA5
ClaI0.2
BamH10.2
KBuffer2
ddH2O 12.6



PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP)5
dNTP5
KOD Plus1
KOD Plus Buffer5
MgSO42
F-Primer1
R-Primer1
ddH2O34


conditions
Temperature(°C)Time Length(0:00:00)
9405:00
9400:3030 cycles
5500:3030 cycles
7201:3030 cycles
7207:00
Alarm5 times
Hold4°C
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