IGEM:Peking University/2008/Experiment/PKU 080512 G1 1

From OpenWetWare

< IGEM:Peking University | 2008 | Experiment(Difference between revisions)
Jump to: navigation, search
(PKU_080512_G1_1)
Current revision (00:46, 12 July 2008) (view source)
 
(9 intermediate revisions not shown.)
Line 3: Line 3:
::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA'''
::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA'''
{|align="right"
{|align="right"
-
[[PKU_photo1.jpg|200px]]
+
[[Image:PKU_080512_ADH-plasmid-check(95min).tif|400px]]
|}
|}
Line 28: Line 28:
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
{|align="right"
{|align="right"
-
[[PKU_20080512_adhpcrcheck.tif|200px]]
+
[[Image:PKU_080512_ADH-PCR-check.tif|200px]]
|}
|}
{|border="1" cellspacing="0"  
{|border="1" cellspacing="0"  
Line 57: Line 57:
|+  
|+  
|-
|-
-
|Temperature(&&&)||Time Length(0:00:00)
+
|Temperature(°C)||Time Length(0:00:00)
|-  
|-  
| 94||05:00
| 94||05:00
Line 71: Line 71:
| Alarm||5 times
| Alarm||5 times
|-
|-
-
| Hold||4&&&
+
| Hold||4°C
 +
|}
 +
 
 +
{|align="right"
 +
|back to [[PKU_Experiment|Previous Page]]
|}
|}

Current revision

PKU_080512_G1_1


Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
Volume (μL)
DNA5
ClaI0.2
BamH10.2
KBuffer2
ddH2O 12.6



PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP)5
dNTP5
KOD Plus1
KOD Plus Buffer5
MgSO42
F-Primer1
R-Primer1
ddH2O34


conditions
Temperature(°C)Time Length(0:00:00)
9405:00
9400:3030 cycles
5500:3030 cycles
7201:3030 cycles
7207:00
Alarm5 times
Hold4°C
back to Previous Page
Personal tools