IGEM:Peking University/2008/Experiment/PKU 080512 G1 1

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(PKU_080512_G1_1)
Current revision (00:46, 12 July 2008) (view source)
 
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::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
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{|align="right"
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[[PKU_20080512_adhpcrcheck.tif|200px]]
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[[Image:PKU_080512_ADH-PCR-check.tif|200px]]
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Current revision

PKU_080512_G1_1


Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
Volume (μL)
DNA5
ClaI0.2
BamH10.2
KBuffer2
ddH2O 12.6



PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP)5
dNTP5
KOD Plus1
KOD Plus Buffer5
MgSO42
F-Primer1
R-Primer1
ddH2O34


conditions
Temperature(°C)Time Length(0:00:00)
9405:00
9400:3030 cycles
5500:3030 cycles
7201:3030 cycles
7207:00
Alarm5 times
Hold4°C
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