IGEM:Peking University/2008/Experiment/PKU 080512 G1 1: Difference between revisions

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(New page: ==PKU_080512_G1_1== ::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA''' {|align="right" 200px |} {|border="1" cellspacing="0" |+ |- | ||Volume (μL) ...)
 
 
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==PKU_080512_G1_1==
==PKU_080512_G1_1==
<hr class=divider>
::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA'''
::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA'''
{|align="right"
{|align="right"
[[PKU_photo1.jpg|200px]]
[[Image:PKU_080512_ADH-plasmid-check(95min).tif|400px]]
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<hr class=divider>


::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
{|align="right"
{|align="right"
[[PKU_20080512_adhpcrcheck.tif|200px]]
[[Image:PKU_080512_ADH-PCR-check.tif|200px]]
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{|border="1" cellspacing="0"  
{|border="1" cellspacing="0"  
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|+  
|+  
|-
|-
|Temperature(&&&)||Time Length(0:00:00)
|Temperature(°C)||Time Length(0:00:00)
|-  
|-  
| 94||05:00
| 94||05:00
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| Alarm||5 times
| Alarm||5 times
|-
|-
| Hold||4&&&
| Hold||4°C
|}
 
{|align="right"
|back to [[PKU_Experiment|Previous Page]]
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Latest revision as of 21:46, 11 July 2008

PKU_080512_G1_1

Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
Volume (μL)
DNA 5
ClaI 0.2
BamH1 0.2
KBuffer 2
ddH2O 12.6


PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP) 5
dNTP 5
KOD Plus 1
KOD Plus Buffer 5
MgSO4 2
F-Primer 1
R-Primer 1
ddH2O 34


conditions
Temperature(°C) Time Length(0:00:00)
94 05:00
94 00:30 30 cycles
55 00:30 30 cycles
72 01:30 30 cycles
72 07:00
Alarm 5 times
Hold 4°C
back to Previous Page
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