IGEM:Peking University/2008/Experiment/PKU 080512 G1 1

From OpenWetWare
Revision as of 08:27, 3 July 2008 by Jiqian Xing (talk | contribs) (New page: ==PKU_080512_G1_1== ::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA''' {|align="right" 200px |} {|border="1" cellspacing="0" |+ |- | ||Volume (μL) ...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

PKU_080512_G1_1

Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
200px
Volume (μL)
DNA 5
ClaI 0.2
BamH1 0.2
KBuffer 2
ddH2O 12.6



PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
200px
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP) 5
dNTP 5
KOD Plus 1
KOD Plus Buffer 5
MgSO4 2
F-Primer 1
R-Primer 1
ddH2O 34


conditions
Temperature(&&&) Time Length(0:00:00)
94 05:00
94 00:30 30 cycles
55 00:30 30 cycles
72 01:30 30 cycles
72 07:00
Alarm 5 times
Hold 4&&&
Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Peking_University/2008/Experiment/PKU_080512_G1_1&oldid=218090"