IGEM:Peking University/2008/Experiment/PKU 080512 G1 1

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PKU_080512_G1_1

Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
200px
Volume (μL)
DNA5
ClaI0.2
BamH10.2
KBuffer2
ddH2O 12.6



PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
200px
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP)5
dNTP5
KOD Plus1
KOD Plus Buffer5
MgSO42
F-Primer1
R-Primer1
ddH2O34


conditions
Temperature(&&&)Time Length(0:00:00)
9405:00
9400:3030 cycles
5500:3030 cycles
7201:3030 cycles
7207:00
Alarm5 times
Hold4&&&
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