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PKU_080512_G1_1
- Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
|
Volume (μL)
|
DNA |
5
|
ClaI |
0.2
|
BamH1 |
0.2
|
KBuffer |
2
|
ddH2O |
12.6
|
- PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
200px
|
Volume (μL)
|
pGREG505/(TEF-lacI-adhlacS-GFP) |
5
|
dNTP |
5
|
KOD Plus |
1
|
KOD Plus Buffer |
5
|
MgSO4 |
2
|
F-Primer |
1
|
R-Primer |
1
|
ddH2O |
34
|
- conditions
Temperature(°C) |
Time Length(0:00:00)
|
94 |
05:00
|
94 |
00:30 |
30 cycles
|
55 |
00:30 |
30 cycles
|
72 |
01:30 |
30 cycles
|
72 |
07:00
|
Alarm |
5 times
|
Hold |
4°C
|