IGEM:Peking University/2008/Experiment/PKU 080513 G1 1: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Jiqian Xing (talk | contribs) |
Jiqian Xing (talk | contribs) |
||
| Line 2: | Line 2: | ||
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul''' | ::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul''' | ||
{|align="right" | {|align="right" | ||
[[PKU_20080513_adhpcrcheck.tif|200px]]|} | [[PKU_20080513_adhpcrcheck.tif|200px]] | ||
|} | |||
{|border="1" cellspacing="0" | {|border="1" cellspacing="0" | ||
|+ | |+ | ||
| Line 22: | Line 23: | ||
|ddH<sub>2</sub>O||36 | |ddH<sub>2</sub>O||36 | ||
|} | |} | ||
| Line 46: | Line 48: | ||
<hr class=divider> | |||
Revision as of 08:34, 3 July 2008
PKU_080513_G1_1
- PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
| Volume (μL) | ||||
| pGREG505/(TEF-lacI-adhlacS-GFP) | 1 | |||
| dNTP | 5 | _ | transtart taq | 1 |
| transtart Buffer | 5 | |||
| F-Primer | 1 | |||
| R-Primer | 1 | |||
| ddH2O | 36 |
- conditions
| Temperature(&&&) | Time Length(0:00:00) | |
| 94 | 05:00 | |
| 94 | 00:30 | 30 cycles |
| 50,55,60 | 00:30 | 30 cycles |
| 72 | 01:30 | 30 cycles |
| 72 | 07:00 | |
| Alarm | 5 times | |
| Hold | 4&&& |
- Enzyme cutting-DNA (pGREG503/504)-SacI-XhoI-20 μL
| Volumn(μL) | ||
| pGREG503/504 | 5 | |
| SacI | 0.2 | |
| XhoI | 0.2 | *5 |
| M Buffer | 2 | |
| 12.6 |
