IGEM:Peking University/2008/Experiment/PKU 080513 G1 1: Difference between revisions

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(New page: ==PKU_080513_G1_1== ::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul''' {|align=”right” 200px|} {|border="1" cellspacing="0" |+ |- | ||Volume (...)
 
 
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==PKU_080513_G1_1==
==PKU_080513_G1_1==
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
<hr class=divider>
{|align=”right”
::'''PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul'''
[[PKU_20080513_adhpcrcheck.tif|200px]]|}
{|align="right"
[[Image:PKU_080513_ADH-PCR-check.tif|400px]]
|}
{|border="1" cellspacing="0"  
{|border="1" cellspacing="0"  
|+  
|+  
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| ||Volume (μL)  
| ||Volume (μL)  
|-  
|-  
| pGREG505/(TEF-lacI-adhlacS-GFP)||1
| pGREG505/(TEF-lacI-ADHlacS-GFP)||1
|-  
|-  
|dNTP||5
|dNTP||5
|_
|-
|transtart taq||1
|transtart taq||1
|-
|-
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|ddH<sub>2</sub>O||36
|ddH<sub>2</sub>O||36
|}
|}




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|+  
|+  
|-
|-
|Temperature(&&&)||Time Length(0:00:00)
|Temperature(°C)||Time Length(0:00:00)
|-  
|-  
| 94||05:00
| 94||05:00
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| Alarm||5 times
| Alarm||5 times
|-
|-
| Hold||4&&&
| Hold||4°C
|}
|}




<hr class=divider>




 
::'''Enzyme cutting-DNA (pGREG503/504)-SacI-XhoI-20 μL'''
::’’’Enzyme cutting-DNA (pGREG503/504)-SacI-XhoI-20 μL’’’
{|align="right"
{|align=”right”
[[Image:PKU_080513_pGREG504-cut.tif|400px]]
[[PKU_20080513_pGREG504.tif|200px]]
|}
|}
{|border="1" cellspacing="0"  
{|border="1" cellspacing="0"  
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| M Buffer||2
| M Buffer||2
|-
|-
|ddH<sub>2</sub>O|12.6
|ddH<sub>2</sub>O||12.6
|}
 
{|align="right"
|back to [[PKU_Experiment|Previous Page]]
|}
|}

Latest revision as of 21:46, 11 July 2008

PKU_080513_G1_1

PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul
Volume (μL)
pGREG505/(TEF-lacI-ADHlacS-GFP) 1
dNTP 5
transtart taq 1
transtart Buffer 5
F-Primer 1
R-Primer 1
ddH2O 36


conditions
Temperature(°C) Time Length(0:00:00)
94 05:00
94 00:30 30 cycles
50,55,60 00:30 30 cycles
72 01:30 30 cycles
72 07:00
Alarm 5 times
Hold 4°C



Enzyme cutting-DNA (pGREG503/504)-SacI-XhoI-20 μL
Volumn(μL)
pGREG503/504 5
SacI 0.2
XhoI 0.2 *5
M Buffer 2
ddH2O 12.6
back to Previous Page
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