IGEM:Peking University/2008/Experiment/PKU 080513 G1 1

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(PKU_080513_G1_1)
(PKU_080513_G1_1)
Line 1: Line 1:
==PKU_080513_G1_1==
==PKU_080513_G1_1==
<hr class=divider>
<hr class=divider>
-
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
+
::'''PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul'''
{|align="right"
{|align="right"
[[PKU_20080513_adhpcrcheck.tif|200px]]
[[PKU_20080513_adhpcrcheck.tif|200px]]
Line 31: Line 31:
|+  
|+  
|-
|-
-
|Temperature(&&&)||Time Length(0:00:00)
+
|Temperature(°C)||Time Length(0:00:00)
|-  
|-  
| 94||05:00
| 94||05:00
Line 45: Line 45:
| Alarm||5 times
| Alarm||5 times
|-
|-
-
| Hold||4&&&
+
| Hold||4°C
|}
|}

Revision as of 12:53, 3 July 2008

PKU_080513_G1_1


PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul
200px
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP)1
dNTP5
transtart taq1
transtart Buffer5
F-Primer1
R-Primer1
ddH2O36


conditions
Temperature(°C)Time Length(0:00:00)
9405:00
9400:3030 cycles
50,55,6000:3030 cycles
7201:3030 cycles
7207:00
Alarm5 times
Hold4°C




Enzyme cutting-DNA (pGREG503/504)-SacI-XhoI-20 μL
200px
Volumn(μL)
pGREG503/504 5
SacI0.2
XhoI 0.2*5
M Buffer2
ddH2O12.6
back to Previous Page
Personal tools