IGEM:Peking University/2008/Experiment/PKU 080513 G1 1

PKU_080513_G1_1

PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul

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	Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP)	1
dNTP	5	_	transtart taq	1
transtart Buffer	5
F-Primer	1
R-Primer	1
ddH2O	36

conditions

Temperature(&&&)	Time Length(0:00:00)
94	05:00
94	00:30	30 cycles
50,55,60	00:30	30 cycles
72	01:30	30 cycles
72	07:00
Alarm	5 times
Hold	4&&&

’’’Enzyme cutting-DNA (pGREG503/504)-SacI-XhoI-20 μL’’’

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	Volumn(μL)
pGREG503/504	5
SacI	0.2
XhoI	0.2	*5
M Buffer	2
12.6