IGEM:Peking University/2008/Experiment/PKU 080513 G1 1

PKU_080513_G1_1

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PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul

	Volume (μL)
pGREG505/(TEF-lacI-ADHlacS-GFP)	1
dNTP	5
transtart taq	1
transtart Buffer	5
F-Primer	1
R-Primer	1
ddH2O	36

conditions

Temperature(°C)	Time Length(0:00:00)
94	05:00
94	00:30	30 cycles
50,55,60	00:30	30 cycles
72	01:30	30 cycles
72	07:00
Alarm	5 times
Hold	4°C

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Enzyme cutting-DNA (pGREG503/504)-SacI-XhoI-20 μL

	Volumn(μL)
pGREG503/504	5
SacI	0.2
XhoI	0.2	*5
M Buffer	2
ddH2O	12.6

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