IGEM:Peking University/2008/Experiment/PKU 080513 G1 1

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PKU_080513_G1_1


PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul
Volume (μL)
pGREG505/(TEF-lacI-ADHlacS-GFP)1
dNTP5
transtart taq1
transtart Buffer5
F-Primer1
R-Primer1
ddH2O36


conditions
Temperature(°C)Time Length(0:00:00)
9405:00
9400:3030 cycles
50,55,6000:3030 cycles
7201:3030 cycles
7207:00
Alarm5 times
Hold4°C




Enzyme cutting-DNA (pGREG503/504)-SacI-XhoI-20 μL
Volumn(μL)
pGREG503/504 5
SacI0.2
XhoI 0.2*5
M Buffer2
ddH2O12.6
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