IGEM:Peking University/2008/Experiment/PKU 080514 G1 1

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(PKU_080514_G1_1)
(PKU_080514_G1_1)
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==PKU_080514_G1_1==
==PKU_080514_G1_1==
<hr class=divider>
<hr class=divider>
-
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
+
::'''PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul'''
{|align="right"
{|align="right"
[[Image:PKU_080514_ADH-PCR-check.tif|400px]]
[[Image:PKU_080514_ADH-PCR-check.tif|400px]]
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| ||Volume (μL)  
| ||Volume (μL)  
|-  
|-  
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| pGREG505/(TEF-lacI-adhlacS-GFP)||1
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| pGREG505/(TEF-lacI-ADHlacS-GFP)||1
|-  
|-  
| easytaq mixture||25 ||*3
| easytaq mixture||25 ||*3

Revision as of 13:00, 3 July 2008

PKU_080514_G1_1


PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul
Volume (μL)
pGREG505/(TEF-lacI-ADHlacS-GFP)1
easytaq mixture25 *3
F-Primer0.5
R-Primer0.5
ddH2O23


conditions
Temperature(°C)Time Length(0:00:00)
9405:00
9400:3030 cycles
50.1,52.2,55.1,58.3,62.201:0030 cycles
7201:3030 cycles
7207:00
Alarm5 times
Hold4°C
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