IGEM:Peking University/2008/Experiment/PKU 080514 G1 1: Difference between revisions
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==PKU_080514_G1_1== | ==PKU_080514_G1_1== | ||
<hr class=divider> | <hr class=divider> | ||
::'''PCR- pGREG505/(TEF-lacI- | ::'''PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul''' | ||
{|align="right" | {|align="right" | ||
[[Image:PKU_080514_ADH-PCR-check.tif|400px]] | [[Image:PKU_080514_ADH-PCR-check.tif|400px]] | ||
| Line 10: | Line 10: | ||
| ||Volume (μL) | | ||Volume (μL) | ||
|- | |- | ||
| pGREG505/(TEF-lacI- | | pGREG505/(TEF-lacI-ADHlacS-GFP)||1 | ||
|- | |- | ||
| easytaq mixture||25 ||*3 | | easytaq mixture||25 ||*3 | ||
Revision as of 10:00, 3 July 2008
PKU_080514_G1_1
- PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul
| Volume (μL) | ||
| pGREG505/(TEF-lacI-ADHlacS-GFP) | 1 | |
| easytaq mixture | 25 | *3 |
| F-Primer | 0.5 | |
| R-Primer | 0.5 | |
| ddH2O | 23 |
- conditions
| Temperature(°C) | Time Length(0:00:00) | |
| 94 | 05:00 | |
| 94 | 00:30 | 30 cycles |
| 50.1,52.2,55.1,58.3,62.2 | 01:00 | 30 cycles |
| 72 | 01:30 | 30 cycles |
| 72 | 07:00 | |
| Alarm | 5 times | |
| Hold | 4°C |
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