IGEM:Peking University/2008/Experiment/PKU 080517 G1 1: Difference between revisions
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Jiqian Xing (talk | contribs) (New page: ==PKU_080512_G1_1== <hr class=divider> ::'''Cultivation- E.Coli(liquid, Amp<sup>+</sup>)''' <hr class=divider> <hr class=divider> ::'''Plasmid Miniprep-pGREG505/(pADH-lac1)''' {|align="...) |
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<hr class=divider> | <hr class=divider> | ||
::'''Plasmid Miniprep-pGREG505/(pADH-lac1)''' | ::'''Plasmid Miniprep-pGREG505/(pADH-lac1)''' | ||
{|align="right" | {|align="right" | ||
[[PKU_20080517_pADH-lac1-SacIXhoI.tif|200px]] | [[PKU_20080517_pADH-lac1-SacIXhoI.tif|200px]] | ||
|} | |||
<hr class=divider> | |||
::'''Lingation-pGREG504/(pADH)-20μL''' | |||
{|border="1" cellspacing="0" | |||
|+ | |||
|- | |||
| ||Volume (μL) | |||
|- | |||
| T-pADH)||5 | |||
|- | |||
| SacI||0.2 | |||
|- | |||
| XhoI||0.2 | |||
|- | |||
| M Buffer||2 | |||
|- | |||
|ddH<sub>2</sub>O||12.6 | |||
|} | |} |
Revision as of 09:16, 3 July 2008
PKU_080512_G1_1
- Cultivation- E.Coli(liquid, Amp+)
- Plasmid Miniprep-pGREG505/(pADH-lac1)
- Lingation-pGREG504/(pADH)-20μL
Volume (μL) | |
T-pADH) | 5 |
SacI | 0.2 |
XhoI | 0.2 |
M Buffer | 2 |
ddH2O | 12.6 |