IGEM:Peking University/2008/Experiment/PKU 080519 G1 1

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(New page: ==PKU_080519_G1_1== <hr class=divider> ::'''Cultivation- E.Coli(liguid-Amp<sup>+</sup>)''' <hr class=divider> ::'''Plasmid Miniprep-pGREG504/pADH''' <hr class=divider> ::'''Enzyme cutting-...)
(PKU_080519_G1_1)
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<hr class=divider>
<hr class=divider>
::'''Cultivation- E.Coli(liguid-Amp<sup>+</sup>)'''
::'''Cultivation- E.Coli(liguid-Amp<sup>+</sup>)'''
 +
<hr class=divider>
<hr class=divider>
::'''Plasmid Miniprep-pGREG504/pADH'''
::'''Plasmid Miniprep-pGREG504/pADH'''
 +
<hr class=divider>
<hr class=divider>
::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
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{|align="right"
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[[PKU_20080512_adhpcrcheck.tif|200px]]
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Revision as of 12:33, 3 July 2008

PKU_080519_G1_1


Cultivation- E.Coli(liguid-Amp+)

Plasmid Miniprep-pGREG504/pADH

Enzyme cutting-pGREG504/pADH-EcoRV
200px
Volume (μL)
pGREG504/pADH5
EcoRV0.2*13
H Buffer2
ddH2O12.8


Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL)
pGREG504/pADH5
SacI0.2*13
BamHI0.2
H Buffer2
ddH2O13.6
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