IGEM:Peking University/2008/Experiment/PKU 080519 G1 1: Difference between revisions
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Revision as of 09:44, 3 July 2008
PKU_080519_G1_1
- Cultivation- E.Coli(liguid-Amp+)
- Plasmid Miniprep-pGREG504/pADH
- Enzyme cutting-pGREG504/pADH-EcoRV
Volume (μL) | ||
pGREG504/pADH | 5 | |
EcoRV | 0.2 | *13 |
H Buffer | 2 | |
ddH2O | 12.8 |
- Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL) | ||
pGREG504/pADH | 5 | |
SacI | 0.2 | *13 |
BamHI | 0.2 | |
H Buffer | 2 | |
ddH2O | 13.6 |
back to Previous Page |