IGEM:Peking University/2008/Experiment/PKU 080519 G1 1

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(PKU_080519_G1_1)
(PKU_080519_G1_1)
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::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
{|align="right"
{|align="right"
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[[PKU_20080512_adhpcrcheck.tif|200px]]
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[[Image:PKU_080519_check-pGREG504-pADH.tif|400px]]
|}
|}
{|border="1" cellspacing="0"  
{|border="1" cellspacing="0"  

Revision as of 12:05, 3 July 2008

PKU_080519_G1_1


Cultivation- E.Coli(liguid-Amp+)

Plasmid Miniprep-pGREG504/pADH

Enzyme cutting-pGREG504/pADH-EcoRV
Volume (μL)
pGREG504/pADH5
EcoRV0.2*13
H Buffer2
ddH2O12.8


Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL)
pGREG504/pADH5
SacI0.2*13
BamHI0.2
H Buffer2
ddH2O13.6


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