IGEM:Peking University/2008/Experiment/PKU 080519 G1 1

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(PKU_080519_G1_1)
(PKU_080519_G1_1)
Line 38: Line 38:
|BamHI||0.2||
|BamHI||0.2||
|-  
|-  
-
|H Buffer||2
+
|K Buffer||2
|-
|-
|ddH<sub>2</sub>O||13.6  
|ddH<sub>2</sub>O||13.6  

Revision as of 06:29, 4 July 2008

PKU_080519_G1_1


Cultivation- E.Coli(liguid-Amp+)

Plasmid Miniprep-pGREG504/pADH

Enzyme cutting-pGREG504/pADH-EcoRV
Volume (μL)
pGREG504/pADH5
EcoRV0.2*13
H Buffer2
ddH2O12.8


Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL)
pGREG504/pADH5
SacI0.2*13
BamHI0.2
K Buffer2
ddH2O13.6


back to Previous Page
Personal tools