IGEM:Peking University/2008/Experiment/PKU 080519 G1 1: Difference between revisions

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(New page: ==PKU_080519_G1_1== <hr class=divider> ::'''Cultivation- E.Coli(liguid-Amp<sup>+</sup>)''' <hr class=divider> ::'''Plasmid Miniprep-pGREG504/pADH''' <hr class=divider> ::'''Enzyme cutting-...)
 
 
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<hr class=divider>
<hr class=divider>
::'''Cultivation- E.Coli(liguid-Amp<sup>+</sup>)'''
::'''Cultivation- E.Coli(liguid-Amp<sup>+</sup>)'''
<hr class=divider>
<hr class=divider>
::'''Plasmid Miniprep-pGREG504/pADH'''
::'''Plasmid Miniprep-pGREG504/pADH'''
<hr class=divider>
<hr class=divider>
::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
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|BamHI||0.2||
|BamHI||0.2||
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|H Buffer||2
|K Buffer||2
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|ddH<sub>2</sub>O||13.6  
|ddH<sub>2</sub>O||13.6  
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Latest revision as of 21:47, 11 July 2008

PKU_080519_G1_1

Cultivation- E.Coli(liguid-Amp+)
Plasmid Miniprep-pGREG504/pADH
Enzyme cutting-pGREG504/pADH-EcoRV
Volume (μL)
pGREG504/pADH 5
EcoRV 0.2 *13
H Buffer 2
ddH2O 12.8


Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL)
pGREG504/pADH 5
SacI 0.2 *13
BamHI 0.2
K Buffer 2
ddH2O 13.6


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