IGEM:Peking University/2008/Experiment/PKU 080519 G1 1: Difference between revisions

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::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
{|align="right"
{|
[[PKU_20080512_adhpcrcheck.tif|200px]]
[[Image:PKU_080519_check-pGREG504-pADH.tif|600px]]
|}
|}
{|border="1" cellspacing="0"  
{|border="1" cellspacing="0"  
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|BamHI||0.2||
|BamHI||0.2||
|-  
|-  
|H Buffer||2
|K Buffer||2
|-
|-
|ddH<sub>2</sub>O||13.6  
|ddH<sub>2</sub>O||13.6  

Latest revision as of 21:47, 11 July 2008

PKU_080519_G1_1

Cultivation- E.Coli(liguid-Amp+)
Plasmid Miniprep-pGREG504/pADH
Enzyme cutting-pGREG504/pADH-EcoRV
Volume (μL)
pGREG504/pADH 5
EcoRV 0.2 *13
H Buffer 2
ddH2O 12.8


Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL)
pGREG504/pADH 5
SacI 0.2 *13
BamHI 0.2
K Buffer 2
ddH2O 13.6


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