IGEM:Peking University/2008/Experiment/PKU 080519 G1 1

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(PKU_080519_G1_1)
Current revision (00:47, 12 July 2008) (view source)
 
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<hr class=divider>
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::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
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{|align="right"
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{|
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[[PKU_20080512_adhpcrcheck.tif|200px]]
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[[Image:PKU_080519_check-pGREG504-pADH.tif|600px]]
|}
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{|border="1" cellspacing="0"  
{|border="1" cellspacing="0"  
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|BamHI||0.2||
|BamHI||0.2||
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|-  
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|H Buffer||2
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|K Buffer||2
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|-
|ddH<sub>2</sub>O||13.6  
|ddH<sub>2</sub>O||13.6  

Current revision

PKU_080519_G1_1


Cultivation- E.Coli(liguid-Amp+)

Plasmid Miniprep-pGREG504/pADH

Enzyme cutting-pGREG504/pADH-EcoRV
Volume (μL)
pGREG504/pADH5
EcoRV0.2*13
H Buffer2
ddH2O12.8


Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL)
pGREG504/pADH5
SacI0.2*13
BamHI0.2
K Buffer2
ddH2O13.6


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