IGEM:Peking University/2008/Experiment/PKU 080519 G1 1: Difference between revisions
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::'''Enzyme cutting-pGREG504/pADH-EcoRV''' | ::'''Enzyme cutting-pGREG504/pADH-EcoRV''' | ||
{| | {| | ||
[[Image:PKU_080519_check-pGREG504-pADH.tif| | [[Image:PKU_080519_check-pGREG504-pADH.tif|600px]] | ||
|} | |} | ||
{|border="1" cellspacing="0" | {|border="1" cellspacing="0" | ||
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|BamHI||0.2|| | |BamHI||0.2|| | ||
|- | |- | ||
| | |K Buffer||2 | ||
|- | |- | ||
|ddH<sub>2</sub>O||13.6 | |ddH<sub>2</sub>O||13.6 |
Latest revision as of 21:47, 11 July 2008
PKU_080519_G1_1
- Cultivation- E.Coli(liguid-Amp+)
- Plasmid Miniprep-pGREG504/pADH
- Enzyme cutting-pGREG504/pADH-EcoRV
Volume (μL) | ||
pGREG504/pADH | 5 | |
EcoRV | 0.2 | *13 |
H Buffer | 2 | |
ddH2O | 12.8 |
- Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL) | ||
pGREG504/pADH | 5 | |
SacI | 0.2 | *13 |
BamHI | 0.2 | |
K Buffer | 2 | |
ddH2O | 13.6 |
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